购彩中心

题目：基于重组蛋白分子设计的生物分析新方法
演讲人：聂舟，湖南大学购彩大厅 教授，优青，杰青，青年长江，青年拔尖获得者
时间：2018年4月23日14：00-
地点：化学楼A楼528室
邀请人：张川 特别研究员

基于重组蛋白分子设计的生物分析新方法
聂舟*
湖南大学购彩大厅

Abstract：
Fluorescent proteins (FPs) have emerged as valuable biological markers for gene expression and protein tracking in living systems. Developing synthetic materials mimicking natural fluorescent proteins is crucial for elucidating photo-physical mechanism of FPs and creating new emission-responsive materials and biosensors. Molecular engineering of FPs and their mimics is an efficient approach to provide new bio-molecular toolkits for biotechnology and bio-analytical chemistry. We reported here a series of new bio-sensing and bio-imaging approaches based on engineered FPs, including ultra-sensitive detection of site-specific DNA methylation, DNA oxidative damage, enzymatic activities of post-translational modification-related enzymes. Moreover, we developed a set of DNA mimics of RFPs with high quantum yields, large Stokes shifts, excellent anti-photobleaching properties, and two-photon fluorescence. The DNA RFP mimics have been exploited for imaging of target membrane proteins in live cells and tissue samples without the need for genetic coding.
 
Short BIOGRAPHY

聂舟，湖南大学购彩大厅 教授，博士生导师，副院长。研究领域为生命分析化学及化学生物学，主要研究方向为功能蛋白质和核酸的生物传感新方法研究。是国家自然科学基金杰出青年基金、中国化学会青年化学奖及卢嘉锡优秀导师奖获得者，并入选教育部“长江学者”青年学者，国家万人计划青年拔尖人才。作为通讯作者在Angew Chem.、 Chem. Sci.、Nucleic Acids Res.、Small、Anal. Chem. Chem. Commun.等期刊发表SCI论文60余篇（影响因子 > 5，48篇），近五年论文他引1800余次。2015年起任英国皇家化学会所属学术期刊《RSC Advances》Associate Editor。